FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727985
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG9450.080.128083
AGTAGCT35750.079.9400945
CTACTCG10800.079.678742
GCTACTC25200.078.3961941
GAGTAGC23700.076.267274
TACTCGG11550.076.133423
CCGAGTA10500.075.029492
GCTGGGA96450.071.670279
GGAGGCT68500.070.960619
TAGCTGG43750.070.2990347
GTAGCTG40850.069.8688056
CCAAAGT65150.062.507592
AAGTGCT59550.061.9369855
ACTCGGG15550.061.2617074
AGTGCTG62400.059.934436
AAAGTGC60050.059.7942164
CCCGAGT15600.058.269941
CAAAGTG65500.058.1752363
TCGGGAG17350.056.725446
TTGGGAG57350.055.773426
Produced by FastQC (version 0.11.5)