FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867497
Sequences flagged as poor quality0
Sequence length35-351
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA9150.099.2906342
TACTCGG8900.092.627883
CGAGTAG8650.091.414983
GTAGCTG30050.081.741766
GCTGGGA72250.081.035829
AGTAGCT29100.080.941395
CCCGAGT11250.080.756381
CTACTCG9000.080.382512
GAGTAGC19450.078.714984
GCTACTC20100.077.006491
TAGCTGG32200.076.806347
AAGTGCT45950.074.326925
AAAGTGC46100.072.6252754
CCAAAGT48850.072.325312
GGAGGCT51000.071.915419
CCCAAAG54500.068.840491
CAAAGTG49950.068.0379943
AGTGCTG50600.066.831496
CTCGGGA13050.065.749935
TCGGGAG13850.064.381596
Produced by FastQC (version 0.11.5)