FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228598
Sequences flagged as poor quality0
Sequence length35-351
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG10700.078.883953
CTACTCG10400.077.913072
CGAGTAG9800.072.347853
AGTAGCT36050.063.2165685
GAGTAGC22350.062.6906784
GTAGCTG35850.062.627486
GCTGGGA88900.062.4736029
CCGAGTA11050.061.1082882
TAGCTGG40950.061.011297
CTCGGGA15400.055.9051555
GCTACTC26150.055.5174061
GGAGGCT61750.055.495989
CCCAAAG66350.055.464871
TCGGGAG15550.055.3658756
AAAGTGC55800.054.455374
CCAAAGT62650.054.1598432
ACTCGGG15650.053.9334344
AAGTGCT58750.053.7323885
CAAAGTG61650.051.204833
AGTGCTG61000.049.813276
Produced by FastQC (version 0.11.5)