FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128545
Sequences flagged as poor quality0
Sequence length35-351
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA9550.0148.526822
CGAGTAG9850.0138.860213
CTACTCG10300.0116.399242
TACTCGG10800.0111.010393
GAGTAGC22000.0110.5271154
CCCGAGT13200.0110.015411
GCTACTC25950.0108.018891
AGTAGCT36550.0106.259925
GTAGCTG39050.0104.213756
TAGCTGG40650.0103.850477
GCTGGGA94350.0102.014529
GGAGGCT64850.091.916569
TCGGGAG14400.087.948376
AAAGTGC59750.084.78374
AAGTGCT62950.082.6197745
CCAAAGT67200.080.158662
CTCGGGA15700.079.590485
CCCAAAG73950.078.322211
CAAAGTG67550.078.2434543
ACTCGGG14750.077.8477254
Produced by FastQC (version 0.11.5)