FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128545
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG6150.0117.495753
GAGTAGC14500.0110.649274
GCTACTC22000.0109.405091
GTAGCTG25850.0107.375276
AGTAGCT24800.0106.177385
CTACTCG10200.0103.6845552
CCGAGTA7050.0103.456352
TAGCTGG28250.0101.746567
GCTGGGA69100.098.701729
TACTCGG10150.096.531543
GGAGGCT50600.091.472159
AAAGTGC43400.090.3036964
AAGTGCT44250.089.399385
CAAAGTG47400.084.233443
CCAAAGT49250.082.933132
AGTGCTG49400.081.159366
CCCAAAG53750.080.2869341
GTGCTGG51800.075.493877
CTCGGGA12900.075.003695
CTACTTG17650.073.694332
Produced by FastQC (version 0.11.5)