FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134358
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9450.0115.727742
TACTCGG9750.0112.989913
CCGAGTA6300.096.439782
GCTACTC20000.092.350741
GTAGCTG23550.092.139026
AGTAGCT23200.090.2214665
CGAGTAG6600.089.0223543
GAGTAGC15050.088.652664
GCTGGGA55800.087.9378749
GGAGGCT45500.084.71599
TAGCTGG26250.083.6012047
TCGGGAG14300.082.766976
ACTCGGG13100.080.357964
AGTGCTG38850.079.336096
CTCGGGA13800.078.0558245
AAAGTGC38200.075.3019564
GTGCTGG41650.074.59467
AAGTGCT40500.073.4434365
CAAAGTG43550.069.986293
CCCAAAG48100.068.9674761
Produced by FastQC (version 0.11.5)