FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032382
Sequences flagged as poor quality0
Sequence length35-251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA5350.094.918072
CGAGTAG5050.091.632863
GGAGGCT29450.078.974289
GCTGGGA42250.078.969899
CCCGAGT8250.071.811751
AGTAGCT21050.071.1560755
AGTGCTG28250.070.771136
GAGTAGC12800.069.4499364
GCTACTC14550.068.9713061
GTAGCTG20700.067.54946
TAGCTGG21200.067.113387
AAGTGCT29650.066.534875
TACTCGG7000.066.106563
GTGCTGG30050.065.307417
CTACTCG6900.064.834482
AAAGTGC30400.063.2910774
CCAAAGT34550.060.191312
TGCTGGG35000.058.101338
CAAAGTG33400.057.9708443
TCGGGAG9000.057.2392276
Produced by FastQC (version 0.11.5)