FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421473
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA9450.092.545452
CGAGTAG9900.081.543553
GAGTAGC24300.073.364024
CCCGAGT13350.073.071081
AGTAGCT42150.070.625135
GCTGGGA94150.069.6671149
GGAGGCT69200.069.50949
TACTCGG11150.069.385153
GTAGCTG43300.069.137826
TAGCTGG47950.068.395827
GCTACTC30400.059.751531
CTACTCG11850.059.6093642
CCAAAGT68750.058.4667242
CCCAAAG75850.058.0957761
TCGGGAG15850.056.238046
CTCGGGA15850.054.1158525
ACTCGGG15950.053.776564
AGTGCTG64300.053.6199576
CAAAGTG69800.053.0091483
AAAGTGC63200.052.6904224
Produced by FastQC (version 0.11.5)