FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875132
Sequences flagged as poor quality0
Sequence length35-351
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA12950.059.3554842
CGAGTAG12550.055.9214363
GCTGGGA110650.055.573689
GGAGGCT73500.049.788569
GAGTAGC30300.049.6332444
CTACTCG14950.048.06182
CCCGAGT17950.047.4764751
TACTCGG14500.047.2485663
GCTACTC34950.046.376431
GTAGCTG55100.046.0961576
AGTAGCT54750.044.8648225
CCCAAAG90200.044.6460761
TAGCTGG59450.044.4097187
AGTGCTG80000.042.819016
CCAAAGT87950.041.0384252
GTGCTGG81600.040.7507557
TCGGGAG20550.040.656596
CGGAGGT17300.040.5672877
AAAGTGC78900.040.0274164
TGCTGGG94350.038.7868
Produced by FastQC (version 0.11.5)