FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875132
Sequences flagged as poor quality0
Sequence length35-251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA74450.059.9801069
CGAGTAG10300.058.060263
CTACTCG10400.057.0771682
GGAGGCT55200.056.804789
CCGAGTA10450.056.8040772
GTAGCTG37400.055.87126
GAGTAGC23050.055.5953524
AGTAGCT37200.054.4609575
TAGCTGG42450.054.4365847
TACTCGG10700.053.6085663
GCTACTC22900.053.430051
CCCGAGT14250.047.6072241
TCGGGAG15500.045.2016036
CTCGGGA15050.043.7902455
AAAGTGC52150.043.294984
ACTCGGG14700.043.1723944
AGCTGGG59500.042.4467288
GTGCTGG55100.041.4927337
AGTGCTG53300.041.2796976
CCAAAGT61200.040.579072
Produced by FastQC (version 0.11.5)