FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003124
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG7300.062.3154563
CCGAGTA7300.062.3154562
GAGTAGC16600.049.7327964
CTCGCGT1702.2009568E-549.5536884
GCTGGGA64300.048.998819
CCCGAGT10050.048.616851
TCGCGTT1455.997642E-446.477945
GTAGCTG31450.045.5358166
AGTAGCT29400.044.6994445
TAGCTGG35300.042.4785967
CCCAAAG53350.039.159951
GTGCTGG46000.038.457977
CCAAAGT47150.036.80532
AGTGCTG44400.035.6697246
AAAGTGC45200.035.411154
CAAAGTG47550.034.0153963
AAGTGCT46200.033.9153145
TGCTGGG55050.033.66598
AGCTGGG54700.029.5691478
TTGGGAG37900.028.895426
Produced by FastQC (version 0.11.5)