FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003124
Sequences flagged as poor quality0
Sequence length35-251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA37400.052.7301949
TAGCTGG19600.052.254247
CTACTCG5750.050.803262
TACTCGG5550.050.7621423
AGTAGCT16250.049.7502985
GTAGCTG17150.049.6460236
GAGTAGC10000.046.5466774
GGAGGCT25850.042.1739
ACTCGGG7550.040.5600174
GCTACTC13050.040.1057051
CTCGGGA6650.038.6814465
CCGAGTA4500.037.8672452
AGTGCTG24550.037.6972166
CGAGTAG4650.036.8790863
TGCTGGG30600.036.827448
GTGCTGG27200.036.292817
AAAGTGC27150.036.0931854
CCCAAAG32550.035.8978541
CCAAAGT28400.035.571972
AGCTGGG28950.035.118398
Produced by FastQC (version 0.11.5)