FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202324
Sequences flagged as poor quality0
Sequence length35-351
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG7900.0104.122213
CCGAGTA8450.0101.318292
GCTGGGA76500.084.264419
GAGTAGC19200.080.437954
GGAGGCT56000.078.539419
TACTCGG9200.078.4612053
GCTACTC24250.078.227451
CCCGAGT12450.078.207981
CTACTCG9300.077.6175462
TAGCTGG35350.075.506117
GTAGCTG32700.074.9513556
AGTAGCT33750.074.609115
CTCGGGA13300.068.157945
GTGCTGG59300.065.9592367
CCCAAAG63700.065.885981
CCAAAGT61600.064.859062
AGTGCTG60750.063.832246
AAAGTGC58450.063.7591974
TCGGGAG14350.063.170786
ACTCGGG13450.062.4053884
Produced by FastQC (version 0.11.5)