FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202324
Sequences flagged as poor quality0
Sequence length35-251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG5650.084.450383
TACTCGG8300.084.019913
GGAGGCT38100.081.245519
TCGGGAG11050.080.29736
CTACTCG8050.078.450832
CCGAGTA6000.076.917012
GAGTAGC13550.072.2330864
AGTAGCT21200.071.560165
GCTGGGA51950.071.125479
TAGCTGG24450.069.555427
CTCGGGA10750.069.423565
GCTACTC17850.068.718421
GTAGCTG23000.067.5108266
ACTCGGG10550.066.1009754
GTGCTGG36050.061.5315677
AGTGCTG35150.061.3540846
AAGTGCT35450.060.7409935
CCAAAGT41150.059.912342
CCCAAAG44500.059.768741
CCCGAGT8450.058.927391
Produced by FastQC (version 0.11.5)