FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379106
Sequences flagged as poor quality0
Sequence length35-251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGCT41550.077.5340969
CCGAGTA5700.076.504532
GCTGGGA51750.075.692799
TACTCGG8550.075.665423
GAGTAGC13000.073.238424
CTACTCG8200.067.9521942
CGAGTAG5950.067.699373
GCTACTC17750.065.513741
TAGCTGG23800.061.9852267
AGTAGCT23550.061.1615945
GTAGCTG23400.060.943316
AGTGCTG40500.060.1027156
AAGTGCT38600.059.76715
TGCTGGG45250.059.598258
GTGCTGG42250.058.7771537
CCCAAAG46500.058.3518451
TCGGGAG12300.057.9704636
CTCGGGA11600.057.8751875
ACTCGGG11750.057.1363564
CCCGAGT8950.056.8441471
Produced by FastQC (version 0.11.5)