FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022655
Sequences flagged as poor quality0
Sequence length35-251
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9500.081.660343
CTACTCG10650.076.805482
CCGAGTA7000.075.611452
GGAGGCT43050.072.366339
CGAGTAG7050.072.212933
GCTGGGA57650.070.230179
CTCGGGA12450.068.152775
GCTACTC22950.067.614521
CCCGAGT9350.066.899781
ACTCGGG12500.064.9714
TCGGGAG13200.064.7532656
GAGTAGC17150.064.317934
AGTGCTG46400.056.842526
TAGCTGG29650.056.8318757
CCCAAAG60500.055.0753561
GTAGCTG29350.054.916576
GTGCTGG47300.054.7283447
AGTAGCT30400.053.43015
AAAGTGC52150.050.670714
TGCTGGG53150.050.632148
Produced by FastQC (version 0.11.5)