FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254684
Sequences flagged as poor quality0
Sequence length35-251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGC13050.068.2405554
CGAGTAG7000.067.96683
GCTGGGA52200.067.289429
CCCAAAG43650.063.5222051
AGTGCTG36600.062.450576
TACTCGG8450.062.078613
AGTAGCT22500.061.2669335
AAAGTGC37450.060.2629174
GGAGGCT38900.059.256649
CTACTCG8200.059.0636752
AAGTGCT38450.058.695615
CCAAAGT42100.057.5204472
GTAGCTG24750.056.6023146
TAGCTGG24900.056.2613337
GCTACTC16800.056.215961
GTGCTGG38900.055.599087
CCGAGTA8150.054.9690782
CAAAGTG42700.054.567713
CCCGAGT9250.052.359151
TGCTGGG45250.052.0191848
Produced by FastQC (version 0.11.5)