FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068105
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA7300.073.625632
CGAGTAG7100.070.968373
GAGTAGC16450.054.114284
GCTGGGA64350.052.984579
AGTAGCT29150.050.7044455
GTAGCTG30900.048.9199456
TAGCTGG32850.048.5724647
CCCGAGT11900.047.9881361
CCAAAGT48450.046.1062472
CCCAAAG52800.044.216341
AAGTGCT44700.043.5865365
AAAGTGC44000.042.371344
GCTACTC19050.042.3202441
CAAAGTG50350.041.3641553
AGTGCTG46000.040.8942386
GTGCTGG51300.038.306327
GGAGGCT48500.037.4010629
TGCTGGG60200.035.15418
CTACTCG7700.034.9004632
TACTCGG7850.034.2335743
Produced by FastQC (version 0.11.5)