FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380015
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA11300.087.3867342
CGAGTAG10850.086.383383
GCTGGGA97250.073.825799
GAGTAGC22900.073.814574
AGTAGCT40450.070.337445
CCCGAGT15250.069.144611
TACTCGG11850.067.794553
GTAGCTG41950.067.423446
TAGCTGG45450.066.64777
AAGTGCT66300.064.874765
CTACTCG11900.064.69682
GCTACTC27750.064.536951
AAAGTGC65800.062.3155444
CCAAAGT68950.062.1409072
CAAAGTG69500.062.130783
CCCAAAG74900.061.6758161
AGTGCTG68600.061.479826
GGAGGCT66350.056.4998789
GTGCTGG75050.055.7480627
TCGGGAG17150.053.672866
Produced by FastQC (version 0.11.5)