FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380015
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG8650.084.754463
CTACTCG9050.084.447162
GCTGGGA57300.078.040849
CCGAGTA8400.076.5402152
GGAGGCT42600.076.0031749
GCTACTC21750.074.179771
CGAGTAG8300.072.1348953
GAGTAGC16600.072.1348954
AGTAGCT25850.069.956585
TAGCTGG28250.068.83217
GTAGCTG27550.067.900726
TCGGGAG11600.065.7788246
AAGTGCT37550.065.405395
AGTGCTG38700.064.874146
AAAGTGC38150.061.1739124
ACTCGGG12600.061.093844
CCCGAGT11250.060.3849371
CCAAAGT44400.058.1955452
GTGCTGG42950.058.1681487
CCCAAAG48250.058.077481
Produced by FastQC (version 0.11.5)