FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936714
Sequences flagged as poor quality0
Sequence length35-351
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA7400.0122.362292
CGAGTAG7950.0107.569373
CTACTCG8200.0104.289822
GCTGGGA67000.098.351319
GAGTAGC17000.097.644694
GCTACTC20650.093.3820341
TACTCGG9550.091.303093
AGTAGCT28150.090.53735
TAGCTGG29700.087.505957
CCCGAGT10550.087.416981
AAAGTGC46000.086.387894
GTAGCTG28800.086.1651466
CCAAAGT51650.084.733622
CCCAAAG57200.082.961391
GGAGGCT47550.082.5140159
AAGTGCT49200.081.450765
CAAAGTG51700.080.7596363
AGTGCTG50800.078.885386
ACTCGGG11300.075.675294
GTGCTGG55250.074.35267
Produced by FastQC (version 0.11.5)