FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936714
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACTC15150.093.0522461
GCTGGGA42650.092.695749
GGAGGCT33000.090.129849
AAGTGCT29100.089.194245
CTACTCG6850.088.7076342
CGAGTAG4150.088.504853
TACTCGG6550.085.9825743
TCGGGAG7700.084.887616
AGTGCTG30600.083.8303156
AAAGTGC30300.083.2374044
GTGCTGG31800.079.115617
AGTAGCT18200.078.70615
CCAAAGT35100.078.595982
CAAAGTG35100.075.691413
CTCGGGA8150.075.111485
CCCAAAG41300.074.1535641
TAGCTGG19750.073.684157
GAGTAGC11450.072.710544
TTGGGAG24450.072.6344766
TGCTGGG36250.072.2573558
Produced by FastQC (version 0.11.5)