FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920655
Sequences flagged as poor quality0
Sequence length35-251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG8050.0100.299353
CTACTCG8400.096.844922
GCTACTC16350.095.797311
GGAGGCT34550.091.337669
GCTGGGA45350.089.814239
AGTAGCT19100.085.186035
AGTGCTG29400.084.255026
GTAGCTG19950.084.012346
AAAGTGC28900.080.4277344
TAGCTGG21600.079.876927
TCGGGAG10200.079.742686
CCGAGTA5350.079.432022
AAGTGCT30700.077.704525
ACTCGGG10150.077.1372154
GAGTAGC12000.076.459264
CGAGTAG5700.075.1178743
CAAAGTG32450.074.2679753
CCAAAGT32700.073.8901752
CTCGGGA10400.072.9303745
GTGCTGG34500.069.656647
Produced by FastQC (version 0.11.5)