FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313767
Sequences flagged as poor quality0
Sequence length35-351
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGC22550.089.511914
CGAGTAG10100.088.267463
GCTGGGA95900.079.982029
AGTAGCT38600.079.746185
CCGAGTA10800.079.4314652
GTAGCTG40950.076.812846
TAGCTGG44000.074.546647
AAAGTGC66200.066.825764
AAGTGCT68200.065.8526155
CCCAAAG79050.065.538271
AGTGCTG70900.065.0055546
CCAAAGT73200.064.571582
CAAAGTG71300.063.69723
GTGCTGG73750.063.633877
GCTACTC25150.063.537741
TACTCGG10250.062.3599623
TGCTGGG82700.059.1879928
CCCGAGT15950.059.0572851
CTACTCG10950.056.8373372
GGAGGCT62400.055.5301369
Produced by FastQC (version 0.11.5)