FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313767
Sequences flagged as poor quality0
Sequence length35-251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA56200.077.590269
GAGTAGC14100.075.586474
AGTAGCT23600.072.1517265
GGAGGCT42900.071.9510049
GCTACTC20700.070.501121
GTAGCTG24050.069.780286
TAGCTGG26250.068.632917
CGAGTAG6450.066.474063
CTACTCG9150.065.126862
TACTCGG9700.061.8825573
AGTGCTG39200.061.384326
GTGCTGG43750.059.7952547
CCAAAGT42550.059.7354132
CCCAAAG47700.059.1499941
AAAGTGC37550.059.0489964
TCGGGAG11500.059.0165446
CCGAGTA7850.058.870682
AAGTGCT39800.058.7887425
CAAAGTG41800.057.1481553
TTGGGAG35700.056.3414846
Produced by FastQC (version 0.11.5)