FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288663
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG8700.079.241853
CCGAGTA8900.075.571842
CCCGAGT13150.060.1006241
GAGTAGC21650.055.91754
AGTAGCT37250.052.8120165
GCTGGGA88450.052.4627089
GTAGCTG37850.050.6421476
TAGCTGG41350.048.3850177
AAAGTGC57550.046.4541974
CCAAAGT62900.046.2472042
AGTGCTG59950.045.7163546
AAGTGCT60200.045.5264975
CCCAAAG71050.044.7305831
GTGCTGG65050.043.6796237
CAAAGTG63250.043.332833
GGAGGCT59650.039.4599959
TGCTGGG77000.038.2108578
GCTACTC24600.036.9118231
CGGGTTC11350.032.5886429
AAGTAGC22250.031.7389414
Produced by FastQC (version 0.11.5)