FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515475
Sequences flagged as poor quality0
Sequence length35-351
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG3900.077.883693
CCGAGTA3900.077.883692
CCCGAGT4950.064.778241
GAGTAGC7900.055.5265354
AGTAGCT14700.049.3520775
GCTGGGA36650.048.7962279
AAGTGCT22550.047.8837055
AAAGTGC21250.047.637254
CGGGTTC4750.046.1746989
AGTGCTG24700.045.0818066
GTAGCTG16100.044.0126726
GCTACTC8250.042.9582021
TAGCTGG17800.042.652737
CCCAAAG28200.042.4903141
CAAAGTG26850.041.4799543
GTGCTGG27650.040.8821757
CCAAAGT27400.040.0314562
TGCTGGG31600.037.907548
GGAGGCT23600.034.3149649
TACTCGG3957.6921424E-834.1768073
Produced by FastQC (version 0.11.5)