FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515475
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG3150.062.149873
CTACTCG3200.060.706522
CGAGTAG2003.2359821E-948.9430243
GCTGGGA23600.047.189549
AGTGCTG14550.046.5915156
GCTACTC6650.045.6439971
ACTCGGG4850.045.411054
AGTAGCT9950.043.0403445
GTGCTGG16550.041.7058647
GTAGCTG10350.041.6806836
TCGGGAG4450.041.5468756
CCGAGTA2659.0403773E-1041.234622
GGAGGCT17050.040.1957369
TAGCTGG12150.038.5491187
TTGGGAG13300.037.9961286
AAGTGCT14700.037.4563985
CTCGGGA5250.037.289925
TGCTGGG19950.036.1930738
GAGTAGC6100.036.105514
CCCAAAG19200.035.4121361
Produced by FastQC (version 0.11.5)