FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618819
Sequences flagged as poor quality0
Sequence length35-351
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA11950.074.6419752
GCTGGGA106900.068.011329
CTACTCG11950.067.600282
CGAGTAG12800.067.055653
TACTCGG12650.065.189963
AGTAGCT47250.060.551135
AAAGTGC73400.058.4681364
AGTGCTG76400.058.154826
GCTACTC29900.057.9750561
GTAGCTG47000.057.2924356
CCCAAAG84250.056.7314071
TAGCTGG51050.056.7032367
GAGTAGC29150.056.5799644
CCCGAGT17900.056.4122471
GGAGGCT77850.056.2069329
AAGTGCT76250.056.062055
CCAAAGT81150.055.165592
CAAAGTG81800.053.904273
GTGCTGG83150.053.6362957
ACTCGGG16900.050.787714
Produced by FastQC (version 0.11.5)