FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618819
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA10300.076.638192
CGAGTAG9950.076.206013
TACTCGG11600.074.855033
GAGTAGC20050.071.366134
AGTAGCT33100.069.831995
CTACTCG11850.068.6634452
GTAGCTG33950.068.217226
GCTGGGA82150.067.736769
GGAGGCT58200.062.830269
TAGCTGG38900.061.120057
GCTACTC27500.060.5002251
AAGTGCT52700.057.3201035
TCGGGAG16850.057.0257036
CCCGAGT13550.056.4638371
AGTGCTG55050.055.49716
AAAGTGC52700.055.463584
CTCGGGA16750.054.030325
ACTCGGG17150.052.7701344
CCCAAAG64900.052.0198631
GTGCTGG59950.051.5775267
Produced by FastQC (version 0.11.5)