FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492121
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG10500.086.296793
CGAGTAG8550.082.855933
GCTGGGA88150.078.30669
GCTACTC25400.076.536671
CCGAGTA10000.075.784272
CTACTCG11750.075.7141652
TAGCTGG40350.073.082977
GAGTAGC20500.069.9152764
GGAGGCT62350.069.2263649
AGTAGCT36950.069.107315
GTAGCTG39000.068.854086
CTCGGGA14950.066.117555
TCGGGAG15800.063.6032686
AAGTGCT66150.061.5139775
ACTCGGG15000.061.5040174
CCCGAGT13800.060.8852841
AAAGTGC65500.060.1122934
CCCAAAG72650.060.094061
AGTGCTG70350.058.3098536
GTGCTGG70800.058.171937
Produced by FastQC (version 0.11.5)