FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492121
Sequences flagged as poor quality0
Sequence length35-251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9700.097.238672
TACTCGG9800.092.012783
GCTACTC21850.091.316371
GGAGGCT52350.077.520119
CTCGGGA12050.075.843225
ACTCGGG13300.073.29614
TCGGGAG13950.072.15326
CCGAGTA7300.071.2298052
GCTGGGA65900.067.498539
CGAGTAG7250.065.549463
GAGTAGC16250.063.7394144
AAAGTGC47100.062.3503154
AAGTGCT48350.061.2424165
AGTAGCT28200.060.9273875
GTAGCTG28650.059.9817736
AGTGCTG49950.058.2409936
CCAAAGT55850.056.727582
TAGCTGG31400.056.292277
CCCAAAG59350.056.2347151
CAAAGTG54950.055.6606833
Produced by FastQC (version 0.11.5)