FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060805
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA7700.066.799542
CGAGTAG7100.065.652893
GCTGGGA74050.063.596959
AGTAGCT29750.061.0528145
AAAGTGC47950.057.322054
AAGTGCT48450.055.7352185
GTAGCTG32550.055.3071486
TAGCTGG34900.055.264927
CCCAAAG58800.054.9456021
CCAAAGT52200.054.8104932
CAAAGTG52500.053.884963
GAGTAGC18200.052.1068534
CTACTCG7800.051.5180742
GCTACTC18100.051.506691
GGAGGCT52000.051.000499
AGTGCTG53250.050.4093486
GTGCTGG57350.049.6059277
TACTCGG8200.049.0049973
CCCGAGT13000.045.748051
TCGGGAG12300.045.7386
Produced by FastQC (version 0.11.5)