FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060805
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG5100.096.933513
CCGAGTA6100.086.345062
TACTCGG6300.080.383893
CTACTCG6500.077.348272
GAGTAGC11500.076.539434
AGTAGCT19950.067.08735
GCTGGGA46950.066.7693569
TAGCTGG22550.065.705277
GGAGGCT34600.063.769719
GTAGCTG22300.062.085036
GCTACTC14000.060.7078671
CCCGAGT10200.058.6792451
TCGGGAG10250.056.8727576
AAGTGCT29650.056.526275
AAAGTGC28350.055.2904974
CCAAAGT32750.054.095992
AGTGCTG32350.051.8073926
CCCAAAG39450.051.584191
CAAAGTG33650.049.4486273
GTGCTGG35500.048.5788157
Produced by FastQC (version 0.11.5)