FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624263
Sequences flagged as poor quality0
Sequence length35-351
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11400.091.715873
TACTCGG11950.091.661043
CTACTCG13000.084.257652
CCGAGTA12350.083.316982
GAGTAGC27950.073.035174
GCTGGGA104150.072.9818659
GCTACTC30400.070.426851
AGTAGCT44100.070.3739245
GTAGCTG46400.067.600926
CCCGAGT17900.065.830551
TAGCTGG52150.063.329727
ACTCGGG18550.062.6271864
GGAGGCT76700.061.6677749
CTCGGGA18600.061.5665665
TCGGGAG19500.058.7250336
CCCAAAG88200.058.5212941
CCAAAGT80600.057.8602182
AAGTGCT76350.055.8641055
AGTGCTG78100.055.4623536
AAAGTGC75000.054.8781174
Produced by FastQC (version 0.11.5)