FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624263
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG10450.087.968922
TACTCGG11300.084.144923
CCGAGTA7800.082.18892
GCTACTC25650.074.036221
GAGTAGC18250.068.799634
CGAGTAG8700.068.657593
GGAGGCT61550.067.148719
GTAGCTG30550.067.123916
GCTGGGA81900.066.8387769
AGTAGCT30950.066.563765
TCGGGAG15900.064.099246
TAGCTGG33250.063.519757
CTCGGGA15800.062.4941945
ACTCGGG16200.060.9511264
AAGTGCT54450.059.999655
AAAGTGC54550.058.772314
AGTGCTG57550.057.395646
CCAAAGT64150.054.8691522
GTGCTGG60300.054.77817
CAAAGTG61500.053.5180663
Produced by FastQC (version 0.11.5)