FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4441306
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA700.00884220426.854963
CGAGTAG14800.025.720883
CCGAGTA15700.024.5648572
GCTGGGA114850.023.3669419
GAGTAGC35650.022.9378264
CTACTCG13000.022.0691952
AGTAGCT57300.021.8987435
GTAGCTG58500.021.7711246
TACTCGG12850.021.5780123
CCCAAAG77850.021.5622331
AAGTGCT68900.021.5541255
AAAGTGC66550.021.2559724
GCTACTC28700.021.1938651
CCAAAGT74250.020.6500532
CGATACG1150.00394446820.4490052
TAGCTGG63650.020.3801547
CAAAGTG75350.019.9585633
CGTCGAG1908.8014E-619.8535771
CCCGAGT21000.019.7590351
AGTGCTG74950.019.7517836
Produced by FastQC (version 0.11.5)