FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3569481
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTT2300.006209394454.017414190-199
CTATACG4256.7258877E-445.958527330-339
CGAGTAG19350.044.8888053
GTTGTCC19507.4324064E-444.460667340-345
CCGAGTA19700.044.4392662
AGTAGCT68950.044.0422255
GTAGCTG70650.042.3543326
GAGTAGC44300.041.7875754
GCTGGGA144850.041.229359
TAGCTGG74750.039.5229037
CCCGAGT23750.039.3357731
AAGTGCT79200.038.902645
CCCAAAG104000.038.2538341
CCAAAGT91500.037.85522
AAAGTGC82500.037.423384
CAAAGTG92200.035.551513
AGTGCTG87450.034.6526226
CCGAGCG5754.5612414E-633.49233270-279
CTATAGC13001.5825208E-1033.345497340-345
TTGGGAG88900.033.231676
Produced by FastQC (version 0.11.5)