FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3569481
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG17950.054.0041283
CCGTACG952.1227606E-951.3456081
CCGAGTA18900.050.9677732
CCGAACG3550.00790103750.820957235-239
AGTAGCT65100.049.1668785
CGAACGT1850.00915448948.967182235-239
GTAGCTG68400.047.2298626
GCTGGGA145250.045.5656479
GAGTAGC43050.045.176424
TAGCTGG74150.043.9747127
CCCGAGT23400.042.2120131
AAGTGCT82500.042.1965035
AGTGCTG83300.042.00146
AAAGTGC84100.040.8862654
CCAAAGT94700.040.8168682
CCCAAAG107100.040.705541
CAAAGTG94450.038.600553
CGTACGT1303.394416E-837.5186352
TTGGGAG92300.036.9813886
GTGCTGG97050.036.926847
Produced by FastQC (version 0.11.5)