FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3065461
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGCG608.8332694E-539.1623425
TACTCGG5300.036.3544773
CGAGTAG7850.035.3209423
CCGAGTA7800.034.9671022
CTACTCG5850.032.95752
CGGGTCG600.004163305731.331419
CCCGAGT9400.030.591941
TCGGGAG7500.029.4500836
GCTGGGA52650.028.8320379
CTCGGGA7900.028.5538125
GAGTAGC19200.027.903174
AGTAGCT30200.026.6096445
CCCAAAG42000.026.6013741
CGTCGAC459.528534E-426.10822956-57
CGGAGGT7850.025.1449537
GGCGGAG10150.025.0021655
ACTCGGG7600.024.7341124
GTGCTGG35350.024.7283257
GTAGCTG31100.024.6307226
GCTACTC14350.023.981481
Produced by FastQC (version 0.11.5)