FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3065461
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT8900.034.8594447
CTACTCG6950.032.4777572
TACTCGG7150.030.9115923
GGCGGAG12350.030.8423635
GCTGGGA54050.030.7824549
CCGAGTA6350.029.62212
GTGCTGG35550.029.6193127
CGAGTAG6300.029.1107673
CCCAAAG50350.028.9580821
GGAGGCT44500.028.094249
GCTACTC17800.028.008731
TTGGGAG42100.027.6949086
AGGCGGA13550.027.4168424
GCGGAGG11000.027.3537986
CTCGGGA8100.027.286165
TGCTGGG41200.027.1507958
CCCGAGT8350.027.0372431
GGGAGGC52350.027.0241838
GGAGGCG13400.026.3200112
AAAGTGC40750.026.0801724
Produced by FastQC (version 0.11.5)