FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3807468
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG6950.030.4498672
CCGAGTA8750.029.5605372
TACTCGG7650.029.4880833
CGACGCG650.006158087428.9210054
CGAGTAG8600.028.4165693
CCCGAGT11600.027.640861
CGCGTAA551.01947924E-425.63486336-37
ACCGCGC3000.025.1477621
GCTGGGA68050.024.6551049
GCTACTC20050.024.4579221
CTCGGGA9500.024.2403685
CCCAAAG56400.023.8268341
TTGGGAG48750.022.751196
GAGTAGC22400.022.6590924
TAGCTGG38450.022.6121647
CGGAGGT10400.022.5945367
GGGAGGC57650.022.581218
GGAGGCT50100.022.5133579
AGTAGCT38400.022.3968325
TAGGCGG2355.2677933E-921.9984255
Produced by FastQC (version 0.11.5)