FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3807468
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG7900.035.12633
CCGAGTA8900.031.1795252
GCTGGGA68650.029.7135249
CCCGAGT11050.027.6751481
GGCGGAG14800.027.3302885
GTGCTGG45250.026.9059857
CCCAAAG58450.026.240491
TTGGGAG53200.026.1607726
TCGCCGT1659.3245035E-825.63779494
GCTACTC21850.025.4079381
CCGAACG1301.0801496E-525.3334031
TGCTGGG53200.024.649558
GGAGGCT51350.024.3470369
CGGAGGT11250.024.235017
AAAGTGC50250.024.1485544
CTACTCG8250.023.3742352
GAGTAGC20650.023.2320544
TAGCTGG40450.023.0098827
AGGCGGA15800.022.9213984
GGGAGGC61600.022.8905858
Produced by FastQC (version 0.11.5)