FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4147314
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1005.2172087E-528.2861791
CGAGTAG10050.027.5902713
CCGAGTA10450.027.0041052
CTACTCG7400.026.0583532
CCCGAGT13350.025.7789131
GCTGGGA69900.024.5406329
CCCAAAG59050.023.6316911
TACTCGG7500.023.1851833
CTCGGGA9600.023.0089455
GAGTAGC25200.021.8200324
CGCAACG1100.00306201121.3622686
GCTACTC21200.021.1256831
AGTAGCT39800.021.0187555
TAGCTGG42750.020.8875527
GTAGCTG40500.020.6554686
GGGAGGC52200.020.5274228
GTGCTGG47350.020.3471667
GGAGGCT51400.020.024019
GATCGCG2400.019.58290726-27
CCAAAGT60900.018.9982072
Produced by FastQC (version 0.11.5)