FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2917769
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG9600.036.225713
CCGAGTA10000.034.805912
CTCGCGT959.4643474E-734.6282774
CCCGAGT12600.032.5737651
GCTGGGA69050.031.9207489
CCCAAAG50000.031.6077841
AGTAGCT35650.031.1106385
GAGTAGC22700.030.0191634
GTAGCTG37550.029.5364636
TAGCTGG38400.029.127937
AAAGTGC41800.028.5570854
CCAAAGT50450.027.8760552
GGAGGCT48250.027.5646829
CGGAGGT9100.027.3714877
CAAAGTG49900.026.0876943
AAGTGCT45650.026.045715
AGTGCTG46800.025.4056956
GTGCTGG46750.025.1317237
CGGGTTC5950.024.4854819
TGCTGGG50700.024.471468
Produced by FastQC (version 0.11.5)