FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2901798
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9900.044.6500053
CTACTCG10000.043.2632
GCTACTC25150.038.897491
CCCGAGT12450.037.0214081
CGAGTAG10800.037.0104183
CCCAAAG60300.036.9706651
CCAAAGT53850.036.8515322
CCGAGTA11650.035.924682
GCTGGGA83050.035.7681589
AAAGTGC48050.035.7213864
TTGGGAG57600.034.6042826
AAGTGCT49200.034.5041165
AGTAGCT38700.034.1447685
TCGGGAG13800.033.7242746
CTCGGGA13800.033.3945665
ACTCGGG13450.033.2146874
AGTGCTG51800.033.1245236
CAAAGTG55400.032.934483
GTAGCTG39600.032.8829776
GAGTAGC24050.032.8490644
Produced by FastQC (version 0.11.5)