FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2961802
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA8700.033.5181162
CTACTCG6550.032.313082
CGAGTAG8050.032.1091843
TACTCGG6850.030.8733943
GAGTAGC20500.030.4901984
CCCGAGT10700.029.9714571
GCTGGGA66150.029.5551559
AGTAGCT35800.028.880335
GTAGCTG34150.028.2114646
GCTACTC18150.027.5430471
GGAGGCT48400.026.1202139
TAGCTGG37450.025.9769617
CCCAAAG50600.025.3513531
AAAGTGC40550.025.2655094
AAGTGCT41850.024.7052735
TTGGGAG43200.024.477176
GGGAGGC54450.023.9947768
AGTGCTG42000.023.9456656
CGGAGGT9050.023.3686547
CCAAAGT47100.023.2670962
Produced by FastQC (version 0.11.5)