FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2961802
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG8000.038.2035683
CCGAGTA8350.037.7284432
TACTCGG8300.037.389223
CTACTCG8400.035.8245932
TCGGGAG10800.033.0801666
GCTACTC21050.032.3949431
CCCAAAG52750.031.5603681
AGTAGCT32300.031.0063275
CTCGGGA11300.030.7911915
GCTGGGA68700.030.7880579
GAGTAGC21650.030.4048864
TTGGGAG49850.029.7045696
GGAGGCT54700.029.6454669
CCCGAGT11050.029.3662221
CGGGTTC6100.029.280629
GGGAGGC64300.028.216488
AAAGTGC44200.028.1901154
TAGCTGG35750.028.005597
GTAGCTG34000.027.7904116
CCAAAGT51500.027.2988592
Produced by FastQC (version 0.11.5)