FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4528595
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG27450.054.746233
CCGAGTA28500.053.855142
AGTAGCT98000.052.3267755
GTAGCTG101950.050.5450676
GAGTAGC66300.048.6362234
GCTGGGA199850.048.3747639
CCCGAGT34350.047.139141
TAGCTGG112850.046.107887
AAGTGCT104950.045.1030045
CGACTAC3658.330426E-443.477425340-345
AAAGTGC110300.043.0856254
CTACTCG22000.042.4296572
CCAAAGT122050.042.2956162
AGTGCTG111950.042.170956
CCCAAAG139200.041.3896871
CAAAGTG121850.040.9046673
GGAGGCT135000.040.676319
TTGGGAG118650.040.3173266
TACTCGG23950.038.9552573
GTGCTGG125150.038.674477
Produced by FastQC (version 0.11.5)