FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4528595
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCG4202.6327009E-587.41219245
CGAGTAG24500.058.637313
ACGTTAG4750.00469398357.968086245
CCGAGTA26100.055.4994742
AGTAGCT87200.052.841675
GAGTAGC58350.050.9768754
TACTCGG27250.050.7513
CTACTCG25650.050.6631552
GTAGCTG93450.049.165346
GCTGGGA199400.048.438329
TAGCTGG104350.045.8520857
TCGGGAG32450.045.542936
CCAAAGT121750.044.995562
CCCGAGT33050.044.736881
AAGTGCT110850.044.6329845
AAAGTGC112700.043.635864
GCTACTC59600.043.6139831
TTGGGAG129150.043.4650766
AGTGCTG117800.042.897756
CTCGGGA33000.042.6296355
Produced by FastQC (version 0.11.5)